The Importance of Knowing Your Cheese Rennet
Rennet is one of the foundational ingredients of the cheesemaking process. Its function may seem simple—coagulating milk—but the type of rennet you choose influences every stage of your cheese’s development, from curd formation to final flavor. Understanding rennet deeply is essential for both home cheesemakers and professional affineurs, as it determines consistency, quality, and the identity of the cheese itself.
This extended guide explores why rennet matters, how different types behave, and how your choices shape the outcome of your cheese.
1. Rennet Is the Architect of Cheese Texture
Curd texture is the backbone of every cheese style. Rennet’s enzymatic action determines:
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How quickly curds form
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How firm the curds become
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How much moisture the curd retains
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The elasticity and stretchability of the final cheese
Animal Rennet: Superior Elasticity and Firmness
Animal rennet contains high levels of chymosin, an enzyme that produces tight, firm, well-structured curds. This is why traditional aged cheeses require animal rennet—it builds a curd that can withstand months or years of aging without collapsing or breaking down.
It is essential for:
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Parmesan & Grana-type cheeses
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Gouda
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Pecorino
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Alpine cheeses (Gruyère, Emmental)
Non-Animal Rennet: Variable Curd Strength
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Microbial rennet produces consistent coagulation but slightly weaker curds.
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Vegetable rennet often produces softer curds and may behave unpredictably depending on the plant source.
Why this matters:
Soft, weak curds release moisture slowly and inconsistently, altering yield and final texture.
2. Rennet Shapes Flavor—Even Long After Curdling
Many cheesemakers underestimate the lasting influence of rennet. After coagulation, rennet enzymes continue breaking down milk proteins throughout aging—a process called proteolysis.
Rennet Choice = Flavor Direction
Animal rennet enhances:
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Nutty notes
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Butteriness
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Meaty and savory umami
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Deep complexity during long aging
Microbial rennet can introduce:
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Mild bitterness over long aging
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Slight “yeasty” or “fermented” notes in some batches
Vegetable rennet contributes:
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Herbaceous, grassy, or earthy notes (from thistle/cardoon)
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Potential bitterness if aged too long
Why this matters:
A cheese aged for six months to two years depends heavily on rennet type for its final flavor development. Choosing the wrong one can produce unexpected off-flavors.
3. Rennet Dictates a Cheese’s Aging Potential
A cheese designed to age needs a strong, resilient protein structure. Rennet helps create this framework.
In Long-Aged Cheeses:
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Curds need to be firm, uniform, and cohesive.
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The enzyme must not break down proteins too aggressively or unevenly.
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Flavor should develop cleanly without bitterness.
Animal rennet or FPC (fermentation-produced chymosin) is ideal because both remain stable and predictable even over long ages.
In Fresh Cheeses:
Curds are not aged long, so rennet choice is more flexible.
Microbial or vegetable rennet works well for:
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Paneer (if rennet is used)
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Farmer’s cheese
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Soft chèvre
Why this matters:
The aging process amplifies any flaws in the rennet. A cheese that tastes fine at 1 month with microbial rennet might turn bitter at 6 months.
4. Rennet Determines Consistency and Predictability in Cheesemaking
A consistent cheese requires consistent rennet.
Animal Rennet Variability
Animal-derived rennet can vary from batch to batch:
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Enzyme strength
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Ratio of chymosin to pepsin
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Seasonal differences in animals’ diets
Professional cheesemakers adjust their recipes to match each batch.
FPC Rennet: The Gold Standard for Precision
Fermentation-produced chymosin is:
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Chemically identical to animal chymosin
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Highly purified
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Stable across temperature ranges
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Extremely consistent in strength
Microbial Rennet: Good but Sometimes Unpredictable
Its behavior can vary with milk type, acidity, and temperature.
Why this matters:
If you want your cheese to taste the same every time, you must choose a rennet that offers consistent coagulation behavior.
5. The Ethics and Dietary Restrictions Behind Rennet Choice
Cheesemakers must consider who will be eating their cheese.
Vegetarian Considerations
Vegetarian consumers avoid animal rennet. Suitable alternatives include:
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Microbial rennet
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Vegetable-derived rennet
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FPC rennet (usually considered vegetarian)
Religious Considerations
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Kosher dairy requires rennet sourced from kosher animals and processed under rabbinical supervision.
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Halal cheese requires rennet sourced from halal-slaughtered animals or non-animal methods.
Traditional Identity
Some cheeses are legally defined by their use of animal rennet:
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Grana Padano
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Manchego
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Pecorino Romano
Why this matters:
Your rennet choice determines both who can enjoy your cheese and whether your cheese is considered authentic within traditional regulations.
6. Rennet Strength Controls Accuracy in Measurement
Different rennets vary in potency.
Using too much rennet:
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Causes bitter taste (due to excess proteolysis)
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Sets curds too fast
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Leads to rubbery, overly firm cheeses
Using too little rennet:
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Produces weak curds that break easily
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Reduces yield
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Causes inconsistent moisture retention
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Leads to overly soft cheese that may not age well
Why this matters:
Understanding rennet strength means you can calculate precise dosages to create stable and predictable curds.
7. Milk Type Responds Differently to Each Rennet
Milk chemistry varies greatly between species.
Cow Milk
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Higher casein micelle stability
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Works with any rennet style
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Very predictable in curd formation
Goat Milk
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Small fat globules
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Softer curd formation
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More delicate protein structure
Sheep Milk
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Very high solids
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Quick to coagulate
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Works exceptionally well with plant rennet (traditional in some Mediterranean cheeses)
Ultra-Pasteurized Milk
Often fails to coagulate properly regardless of rennet.
Why this matters:
The rennet must be matched to the milk for best results.
8. Rennet Influences Yield and Moisture Levels
A strong, clean coagulation means:
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Higher cheese yield
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Better moisture control
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Slower whey expulsion for soft cheeses
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Faster whey release for firm and aged cheeses
Poor rennet choice leads to:
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Crumbly curds
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Low moisture cheeses unintentionally
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Reduced final weight
Why this matters:
The economics of cheesemaking depend on yield—choosing the wrong rennet directly affects profitability and waste.
9. Rennet Complements (or Conflicts With) Cultures
Rennet and cultures work together during curd formation.
Some cultures produce acids that speed up rennet action
→ Good for farmhouse cheddar, feta, and blue cheese.
Some cultures produce enzymes that compete with rennet
→ Can weaken the curd during long aging if paired with a weak rennet.
Why this matters:
Rennet must be chosen to harmonize with the target bacteria and the desired speed of coagulation.
10. Rennet Impacts the Entire Cheesemaking Workflow
Choosing the right rennet affects:
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Setting time
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Cutting time
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Stirring intensity
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Cooking temperature
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Molding pressure
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Salt absorption
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Aging strategy
Every step of the cheesemaking process is influenced by how the curd was formed.
A well-chosen rennet makes the entire process smoother and more predictable.
⭐ Conclusion: Why Knowing Your Rennet Truly Matters
Rennet is not a simple additive—it’s a complex enzyme that determines:
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Texture
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Flavor
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Aroma
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Aging potential
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Shelf life
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Consistency
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Yield
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Traditional authenticity
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Consumer suitability (vegetarian, kosher, halal)
When you understand your rennet, you gain control over the entire cheesemaking process. Knowing your rennet transforms cheesemaking from guesswork into craft, and from craft into mastery.
Essentially there are 5 types of coagulants used to make cheese:
1. Animal Derived Rennet:
This the rennet used in most traditional cheese making plants. The rennet or rennin refers to collection of enzymes that comes from the fourth stomach of ruminant animals (kid, calf or lamb). If we look at rennet through a chemist’s eyes, rennet is chiefly made up of two enzymes that break down protein chains in milk: chymosin and pepsin.
2. Microbial Rennet:
Microbial rennet is term used to describe a coagulating agent produced by a specific type of mold, fungus or yeast organism grown and fermented in a lab setting. This coagulant is considered vegetarian friendly as the enzyme produced by the organism is not derived from an animal.
3. FPC-Fermentation Produced Chymosin Rennet:
FPC Rennet and GMO Controversy
FPC rennet is a GMO product. And, according to the culture companies, 90% of all cheeses produced in North America is made with FPC rennet. However, ingredient labels do not distinquish between this type of microbial rennet or the original non-GMO based type. And the fact that use of FPC type microbial rennet is not labeled a GMO product leaves those who oppose the use of GMOs in the dark when it comes to choosing their cheeses.
In addition, further confusion and debate arises over the general differences between GMO products versus “genetically engineered” products as the latter elicits deeper concerns from those opposed to this type of science.
In the end, what this means is that most cheese in North America is made from vegetarian friendly but still animal originated, GMO derived FPC rennet. And that while use of this type of rennet is banned in GMO-free European countries doesn’t mean the cheese we buy from those countries are necessarily FPC free.
4. Vegetable Rennet:
5. Citric Acid or Vinegar:
Finally, some cheeses like Ricotta are coagulated using simple lemon juice or vinegar. However, this coagulant is mostly used when making a heat precipitated curd. These coagulants are decidedly vegetarian. But this coagulant has a very limited use due to its limitations and noticeable taste profile.




